NanoMass Core

The NanoMass Core provides nano-micro-analytical services measure chemicals with trace sensitivity using unique capillary electrophoresis (CE) electrospray ionization (ESI) high-resolution mass spectrometry (HRMS) instruments that have been custom-built and carefully validated in the Nemes Research Laboratory. These instruments enable the detection of proteins, peptides, metabolites, and man-made chemicals in limited samples (e.g., 250 nL–10 μL volume) that are too small for analysis using standard nanoliquid chromatography (nanoLC) HRMS. A comprehensive set of ancillary instruments are also available to process samples and to analyze complex mass spectrometry data that result from such analyses. Additionally, the core also provides consultative services to help design and execute experiments.

INSTRUMENTATION

1/ Large-molecular CE-nanoESI-HRMS consisting of: 1 custom-built microanalytical CE platform, a custom-built micro/nano-regime sheath-flow CE-ESI interface, and a quadrupole-ion trap-orbitrap tribrid multistage ultra-high resolution mass spectrometer (model Orbitrap Fusion Lumos, ThermoScientific):

  • Recommended for large molecules (Mw > 500 g/mol): Proteins, peptides, larger metabolites

    Large-molecular CE-nanoESI-HRMS
  • Compatibility with limited samples: >~100 nL required
  • Trace-level sensitivity: 210 zmol (156,000 copies) for model peptides
  • Quantification: 4-5 log-order linear dynamic range; relative quantification using TMT/iTRAQ barcoding; absolute quantification using isotopic labeling (e.g., iBAQ)
  • Fee:
    • UMD Rate: $100/h for instrument time, assistance, and data analysis (charged in hourly increments)
    • External Non-Profit/University: $125/h for instrument time, assistance, and data analysis (charged in hourly increments)
    • Commercial: $150/h for instrument time, assistance, and data analysis (charged in hourly increments)
  • Representative studies using this platform:
    • R. M. Onjiko, E. P. Portero, S. A. Moody, and P. Nemes*, In situ microprobe single-cell capillary electrophoresis mass spectrometry: Metabolic reorganization in single differentiating cells in the live vertebrate (X. laevis) embryo, Anal. Chem. 2017, 89, 7069–7076, DOI: 10.1021/acs.analchem.7b00880, PMID: 28434226, Download. Featured publication on the cover page of Analytical Chemistry 2017.
    • R. M. Onjiko, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo, Proc. Nat. Acad. Sci. USA 2015, 112, 6545–6550, DOI: 10.1073/pnas.1423682112, PMID: 25941375, Download (open-access)

2/ Small-molecular CE-microESI-HRMS consisting of: 1 custom-built microanalytical CE platform, a custom-built micro-regime shealth-flow CE-ESI interface, and a quadrupole time-of-flight high-resolution tandem mass spectrometer (model Impact HD, Bruker Daltonics):

Small-molecular CE-microESI-HRMS
  • Recommended for small molecules and peptides (Mw < ~1,000 g/mol)
  • Trace-level sensitivity: 60 amol for model metabolites
  • Quantification: 4-5 log-order linear dynamic range; relative quantification; absolute quantification using external calibration or isotopically labeled standards
  • Fee:
    • UMD Rate: $100/h for instrument time, assistance, and data analysis (charged in hourly increments)
    • External Non-Profit/University: $125/h for instrument time, assistance, and data analysis (charged in hourly increments)
    • Commercial: $150/h for instrument time, assistance, and data analysis (charged in hourly increments)
  • Representative studies using this platform:
    • S. B. Choi, C. Lombard-Banek, P. Muñoz-LLancao, M. C. Manzini, and P. Nemes*, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, accepted, DOI: 10.1007/s13361-017-1838-1, PMID: 29147852, Download
    • C. Lombard-Banek, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry for discovery proteomics: quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo, Angew. Chem. Int. Ed. 2016, 55, 2454–2458, DOI: 10.1002/anie.201510411, Download (open-access)

3/ Sample Processing

  • Spectrophotometer (microanalytical, multiwell-plate): Protein, peptide, or RNA/DNA assays
  • Vacuum concentrator
  • Microanalytical balance
  • Research-grade stereomicroscope equipped with bright-field and 3-channel fluorescence (RGB)
  • Research-grade inverted microscope equipped with bright-field and 3-channel fluorescence (RGB)

4/ Data Processing

  • For proteomics: Proteome Discoverer (Thermo), ProteinScape (Bruker), MaxQuant with licensed copies of SEQUEST (Thermo) and ProteinScape (Bruker) search engines
  • For metabolomics: DataAnalysis (Bruker)

5/ Consultation

  • Experimental design
  • Sample processing
  • Data analysis
  • Publications

CONTACT TO NANOMASS STAFF

  • Contact: Vi Quach, NanoMass Core Operations Manager
  • Email: nanomass[at]umd[dot]edu
  • Phone: 301-405-8612 (Nemes Lab)
  • In your email, please describe briefly the types of molecules (structure, formula, and mass) and amounts of material (e.g., microgram of protein or metabolites extracted from cells) that need analysis to help us determine the most appropriate type of instrumentation and to assign a Staff Member for the request.  Once assigned, the Staff Member will follow up on the request.
  • Instrument bookings are managed by Mr. Quach via the UMD NanoCenter: Nanocenter.umd.edu/scheduler