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[54] N. Drouin*, M. van Mever, W. Zhang, E. Tobolkina, S. Ferre, A-C. Servais, M-J. Gou, L. Nyssen, M. Fillet, G. S. M. Lageveen-Kammeijer, J. Nouta, A. J. Chetwynd, I. Lynch, J. A. Thorn, J. Meixner, C. Lößner, M. Taverna, S. Liu, N. T. Tran, Y. Francois, A. Lechner, R. Nehmé, G. Al Hamoui Dit Banni, R. Nasreddine, C. Colas, H. H. Lindner, K. Faserl, C. Neusüß, M. Nelke, S. Lämmerer, C. Perrin, C. Bich, C. Barbas, Á. López-Gonzálvez, A. Guttman, M. Szigeti, P. Britz-McKibbin, Z. Kroezen, M. Shanmuganathan, P. Nemes, E. P. Portero, T. Hankemeier, S. Codesido, V. González-Ruiz, S. Rudaz, R. Ramautar*, Capillary electrophoresis-mass spectrometry at trial by metabo-ring: Effective electrophoretic mobility for reproducible and robust compound annotation, Anal. Chem. 2020, in print,


[53] Lombard-Banek, Sam B. Choi, and P. Nemes*, Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry, Methods in Enzymology: Enzymatic Activity in Single Cells, Elsevier 2019, Volume 628, 263–, 292, Eds. M. Kovarik and N. L. Albritton, ISSN 0076-6879, DOI: 10.1016/bs.mie.2019.07.001, Download

[52] T. Evers, M. Hochane, S. Tans, R. Heeren, S. Semrau, P. Nemes, and A. Mashaghi*, Deciphering metabolic heterogeneity by single-cell analysis, Anal. Chem. 2019, in print, 10.1021/acs.analchem.9b02410, Download

[51] Z. Liu, E. P. Portero, Y. Jian, Y. Zhao, R. M. Onjiko, C. Zeng*, and P. Nemes*, Trace, Machine learning of signal images for trace-sensitive mass spectrometry: A case study from single-cell metabolomics, Anal. Chem. 2019, 91, 5768–5776, DOI: 10.1021/acs.analchem.8b05985, Download

[50] C. Lombard-Banek, Z. Yu, A. P. Swiercz, P. Marvar, and P. Nemes*, Response to Letter to the Editor regarding “A microanalytical capillary electrophoresis mass spectrometry assay for quantifying angiotensin peptides in the brain,” Anal. Bioanal. Chem. 2019, in print, DOI: 10.1007/s00216-019-02164-8

[49] C. Lombard-Banek, Z. Yu, A. P. Swiercz, P. Marvar, and P. Nemes*, Microanalytical characterization of the renin angiotensin system in identified mouse brain punches, Anal. Bioanal. Chem. 2019, DOI: 10.1007/s00216-019-01771-9

[48] C. Lombard-Banek, S. A. Moody, M. Chiara Manzini, and P. Nemes*, Microsampling capillary electrophoresis mass spectrometry enables single-cell proteomics in complex tissues: developing cell clones in live Xenopus laevis and zebrafish embryos, Anal. Chem. 2019, in print, DOI: 10.1021/acs.analchem.9b00345, Download

[47] P. Portero and P. Nemes, Dual cationic–anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS, Analyst 2019, 144, 892–900, DOI: 10.1039/C8AN01999A, Download


[46] R. M. Onjiko, E. P. Portero, and P. Nemes*, Single-cell metabolomics with capillary electrophoresis mass Spectrometry, Chapter 10, in Capillary Electrophoresis-Mass Spectrometry Approaches for Metabolomics (Ed. R. Ramautar), Royal Society of Chemistry 2018, pp 209–224, ISBN: 978-1-78801-104-4, DOI: 10.1039/9781788012737, Download

[45] A. B. Baxi, C. Lombard-Banek, S. A. Moody, and P. Nemes*, Proteomic characterization of the neural ectoderm fated cell clones in the Xenopus laevis embryo by high-resolution mass spectrometry, ACS Chem. Neurosci. 2018, 9, 2064–2073, DOI: 10.1021/acschemneuro.7b00525, PMID: 29578674, Download


[44] S. B. Choi, C. Lombard-Banek, P. Muñoz-LLancao, M. C. Manzini, and P. Nemes*, Enhanced peptide detection toward single-neuron proteomics by reversed-phase fractionation capillary electrophoresis mass spectrometry, J. Am. Soc. Mass Spectrom. 2017, accepted, DOI: 10.1007/s13361-017-1838-1, PMID: 29147852, Download

[43] R. M. Onjiko, E. P. Portero, S. A. Moody, and P. Nemes*, Microprobe capillary electrophoresis mass spectrometry for single-cell metabolomics in live frog (Xenopus laevis) embryos, J. Vis. Exp. 2017, e56956, DOI: doi:10.3791/56956, PMID: 29286491, Download

[42] R. M. Onjiko, D. O. Plotnick, S. A. Moody, and P. Nemes*, Metabolic comparison of dorsal versus ventral cells directly in the live 8-cell frog embryo by microprobe single-cell CE-ESI-MS, 2017, Anal. Methods, 2017, 9, 4964–4970, DOI: 10.1039/C7AY00834A, PMID: 29062391, Download

[41] R. M. Onjiko, E. P. Portero, S. A. Moody, and P. Nemes*, In situ microprobe single-cell capillary electrophoresis mass spectrometry: Metabolic reorganization in single differentiating cells in the live vertebrate (X. laevis) embryo, Anal. Chem. 2017, 89, 7069–7076, DOI: 10.1021/acs.analchem.7b00880, PMID: 28434226, Download. Featured publication on the cover page of Analytical Chemistry 2017.

[40] J. Li, P. Nemes, and J. Guo, Mapping intermediate degradation products of poly(lactic-co-glycolic acid) (PLGA) in vitro, J. Journal of Biomedical Materials Research: Part B – Applied Biomaterials, 2017, in print, DOI: 10.1002/jbm.b.33920, PMID: 28514061, Download


[39] C. Lombard-Banek, E. P. Portero, R. M. Onjiko, and P. Nemes*, New-generation mass spectrometry expands the toolbox of cell and developmental biology, genesis 2016, 55, e23012, DOI: 10.1002/dvg.23012, Download (open-access)

[38] B. S. Choi, M. Zamarbide, M. Chiara Manzini, and P. Nemes*, Tapered-tip capillary electrophoresis nanoelectrospray ionization for ultrasensitive proteomics: The mouse cortex. J. Am. Soc. Mass Spectrom. 2016, 28, 597–607, DOI: 10.1007/s13361-016-1532-8, Download (open-access). Invited Contribution to the 2016 Emerging Investigator Issue of JASMS.

[37] C. Lombard-Banek, Sally A. Moody, and P. Nemes*, High-sensitivity mass spectrometry for probing gene translation in single embryonic cells in the early frog (Xenopus) embryo, Frontiers in Cell and Dev. Biol. 2016, 4, Article no. 100, DOI: 10.3389/fcell.2016.00100, Download (open access)

[36] C. Lombard-Banek, Sally A. Moody, and P. Nemes*, Label-free quantification of proteins in single embryonic cells with neural fate in the cleavage-stage frog (Xenopus laevis) embryo using capillary electrophoresis electrospray ionization high resolution mass spectrometry (CE-ESI-HRMS), Mol. Cell. Prot. 2016, 15, 2756–2768, DOI: 10.1074/mcp.M115.057760, Download (open access). Highlighted paper by the Editorial Board of Mol. Cell. Prot. Featured on the cover page of Mol. Cell. Prot. 2016.

[35] R. M. Onjiko, S. Morris, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry with multi-solvent extraction identifies metabolic differences between left and right blastomeres in the 8-cell frog (Xenopus) embryo, Analyst 2016, 141, 3648–3656, DOI: 10.1039/C6AN00200E, Download. Featured publication on the cover page of Analyst 2016. Highlighted contribution in the Young Investigator Issue.

[34] C. Lombard-Banek, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry for discovery proteomics: quantifying translational cell heterogeneity in the 16-cell frog (Xenopus) embryo, Angew. Chem. Int. Ed. 2016, 55, 2454–2458, DOI: 10.1002/anie.201510411, Download (open-access)

[33] B. Shrestha, C. M. Walsh, G. R. Boyce, and P. Nemes*, Microprobe mass spectrometry imaging of live tissues, cells, and bacterial colonies using laser ablation electrospray ionization, in Advances in MALDI and Laser-induced soft ionization mass spectrometry, Springer 2016, Ed. R. Cramer, pp. 149–167, Springer International Publishing Switzerland 2016, ISBN 978-3-319-04818-5, DOI: 10.1007/978-3-319-04819-2, Download


[32] H. Li, S. Wickramasekara, and P. Nemes*, One-hour screening of adulterated heparin by simplified peroxide digestion and fast RPIP-LC-MS2, Anal. Chem. 2015, 87, 8424–8432, DOI: 10.1021/acs.analchem.5b01788, PMID: 26168275, Download

[31] R. M. Onjiko, S. A. Moody, and P. Nemes*, Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo, Proc. Nat. Acad. Sci. USA 2015, 112, 6545–6550, DOI: 10.1073/pnas.1423682112, PMID: 25941375, Download (open-access)

[30] H. Li, B. K. Smith, L. Mark, P. Nemes, J. Nazarian, and A. Vertes, Ambient molecular imaging by laser ablation electrospray ionization mass spectrometry with ion mobility separation, Int. J. Mass Spectrom. 2015, 377, 681–689, DOI: 10.1016/j.ijms.2014.06.025, Download

[29] R. Shroff, K. Schramm, P. Nemes, A. Vertes, J. Gershenzon, and A. Svatos, Quantification of plant surface metabolites by MALDI mass spectrometry imaging: glucosinolates on Arabidopsis thaliana leaves, The Plant Journal 2015, 81, 961–972, DOI: 10.1111/tpj.12760, PMID: 25600688, Download (open-access)

[28] P. Nemes* and A. Vertes*, Laser ablation electrospray ionization mass spectrometry: mechanisms, configurations and imaging applications, in Ambient Ionization Mass Spectrometry, New Developments in Mass Spectrometry No. 2, Royal Society of Chemistry, Cambridge, 2015, Eds. M. Domin and R. Cody, Chapter 14, pp. 348–371; ISBN 1849739269; DOI: 10.1039/9781782628026-00348, Download


[27] E. J. Lanni, S. J. B. Dunham, P. Nemes, S. S. Rubakhin, J. V. Sweedler, Biomolecular imaging with a C60-SIMS/MALDI dual ion source hybrid mass spectrometer: Instrumentation, matrix enhancement and single cell analysis, J. Am. Soc. Mass Spectrom. 2014, 11, 1897–1907, DOI: 10.1007/s13361-014-0978-9, PMID: 25183225, Download


[26]  P. Nemes*, W. J. Hoover, and D. A. Keire, Rapid differentiation of heparin from other glycosaminoglycans by pyrolysis mass spectrometry, Anal. Chem. 2013, 85, 7405–7412, DOI: 10.1021/ac401318q, PMID: 23841449, Download

[25] P. Nemes, S. S. Rubakhin, J. Aerts, and J. V. Sweedler, Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry, Nat. Protoc. 2013, 8, 783–799, DOI: 10.1038/nprot.2013.035, PMID: 23538882, Download (open-access). Featured article on metabolomics in the March issue of Nature Protocols 2013.

[24] A. M. Knolhoff, K. M. Nautiyal, P. Nemes, S. Kalachikov, I. Morozova, R. Silver, and J. V. Sweedler, Combining small-volume metabolomic and transcriptomic approaches for assessing brain chemistry, Anal. Chem. 2013, 85, 3136–3143, DOI:, PMID: 23409944, Download (open-access)

[23] A. M. Knolhoff, P. Nemes, S. S. Rubakhin, and J. V. Sweedler, MS-based methodologies for single-cell metabolite detection and identification, in Methodologies for Metabolomics 2013, pp. 119–139, Experimental Strategies and Techniques, Eds. R. Wevers, N. Lutz, and J. V. Sweedler, Cambridge University Press, Download

[22] A. Vertes, B. Shrestha, and P. Nemes, Direct metabolomics from tissues and cells: new approaches for small molecule and lipid characterization, in Methodologies for Metabolomics, Experimental Strategies and Techniques, Cambridge University Press 2013, Eds. R. Wevers, N. Lutz, and J. V. Sweedler, pp. 140–158; ISBN: 9780521765909


[21] P. Nemes, A. M. Knolhoff, S. S. Rubakhin, and J. V. Sweedler, Single-cell metabolomics: changes in the metabolome of freshly isolated and cultured neurons, ACS Chem. Neuro. 2012, 3, 782–792, DOI: 10.1021/cn300100u, PMID: 23077722, Download (open-access). Featured on the 2012 October cover of ACS Chemical Neuroscience

[20] P. Nemes* and A. Vertes, Ambient mass spectrometry for in vivo local analysis and in situ molecular tissue imaging, TrAC-Trends in Analytical Chemistry 2012, 34, 2234, DOI: 10.1016/j.trac.2011.11.006, Download

[19] P. Nemes, H. Huang, and A. Vertes, Internal energy deposition and ion fragmentation in atmospheric-pressure mid-infrared laser ablation electrospray ionization, Phys. Chem. Chem. Phys. 2012, 14, 25012507, DOI: 10.1039/C2CP23411D, PMID: 22249858, Download


[18] S. S. Rubakhin, E. V. Romanova, P. Nemes, and J. V. Sweedler, Profiling metabolites and peptides in single cells, Nat. Methods 2011, 8, S20S29, DOI: 10.1038/nmeth.1549, PMID: 21451513, Download. Featured in a special issue of Nature Methods 2011.

[17] P. Nemes, A. M. Knolhoff, S. S. Rubakhin, and J. V. Sweedler, Metabolic differentiation of neuronal phenotypes by single-cell capillary electrophoresis electrospray ionization mass spectrometry, Anal. Chem. 2011, 83, 68106817, DOI: 10.1021/ac2015855, PMID: 21809850, Download (open-access). Highlighted in a virtual issue featuring 30 articles on advances in modern metabolomics.


[16] P. Nemes and A. Vertes, Atmospheric-pressure molecular imaging of biological tissues and biofilms by LAESI mass spectrometry, Journal of Visualized Experiments 2010, 43, e2097, DOI: 10.3791/2097, PMID: 20834223, Download (open-access). Available in video format at

[15] P. Nemes, A. S. Woods, and A. Vertes, Simultaneous small metabolite and lipid imaging in rat brain tissues and atmospheric pressure by laser ablation electrospray ionization mass spectrometry, Anal. Chem. 2010, 82, 982988, DOI: 10.1021/ac902245p, PMID: 20050678, Download

[14] B. Shrestha, P. Nemes, and A. Vertes, Ablation and analysis of small cell populations and single cells by consecutive laser pulses, Appl. Phys. A 2010, DOI: 10.1007/s00339-010-5781-2, Download

[13] B. Shrestha, P. Nemes, J. Nazarian, Y. Hathout, E. P. Hoffman, and A. Vertes, Direct analysis of lipids and other metabolites in mouse brain tissue by AP IR-MALDI and reactive LAESI mass spectrometry, Analyst 2010, 135, 751758, DOI: 10.1039/B922854C, PMID: 20349540, Download. Featured on the April cover of Analyst 2010.

[12] P. Nemes and A. Vertes, Laser ablation electrospray ionization for atmospheric pressure molecular imaging mass spectrometry, Methods in Molecular Biology: Mass Spectrometry Imaging, Principles and Protocols, Humana Press 2010, Eds. S. S. Rubakhin and J. V. Sweedler, Chapter 10, pp. 159171; ISBN 978-1-60761-745-7; DOI: 10.1007/978-1-60761-746-4_9, Download


[11] P. Nemes, A. A. Barton, and A. Vertes, Three-dimensional imaging of metabolites in tissues under native conditions by laser ablation electrospray ionization mass spectrometry, Anal. Chem. 2009, 81, 66686675, DOI: 10.1021/ac900745e, PMID: 19572562, Download


[10] P. Nemes, A. A. Barton, Y. Li, and A. Vertes, Ambient molecular imaging and depth profiling of live tissue by infrared laser ablation electrospray ionization mass spectrometry, Anal. Chem. 2008, 80, 45754582, DOI: 10.1021/ac8004082, PMID: 18473485, Download

[9] A. Vertes, P. Nemes, B. Shrestha, A. A. Barton, Z. Chen, and Y. Li, Molecular imaging by mid-IR laser ablation mass spectrometry, Appl. Phys. A 2008, 93, 885891, DOI: 10.1007/s00339-008-4750-5, Download

[8] P. Nemes, S. Goyal, and A. Vertes, Conformational and noncovalent complexation changes in proteins during electrospray ionization, Anal. Chem. 2008, 80, 387395, DOI: 10.1021/ac0714359, PMID: 18081323, Download


[7] P. Nemes and A. Vertes, Laser ablation electrospray ionization for atmospheric pressure, in vivo, and imaging mass spectrometry, Anal. Chem. 2007, 79, 80988106, DOI: 10.1021/ac071181r, PMID: 17900146, Download. Reported by more than 15 media channels worldwide upon publication. With 350+ citations, this paper is a “Highly Cited Paper”

[6] P. Nemes, I. Marginean, and A. Vertes, Spraying mode effect on droplet formation and ion chemistry in electrosprays, Anal. Chem. 2007, 79, 31053116, DOI: 10.1021/ac062382i, PMID: 17378541, Download. Featured on the April cover of Analytical Chemistry 2007

[5] I. Marginean, P. Nemes, and A. Vertes, Astable regime in electrosprays, Phys. Rev. E 2007, 76, 026320, DOI: 10.1103/PhysRevE.76.026320, PMID: 17930155, Download


[4] I. Marginean, P. Nemes, L. Parvin, and A. Vertes, How much charge is there on a pulsating Taylor cone? Appl. Phys. Lett. 2006, 89, 064104, DOI: 10.1063/1.2266889, Download

[3] I. Marginean, P. Nemes, and A. Vertes, Oder-chaos-order transitions in electrosprays: The electrified dripping faucet, Phys. Rev. Lett. 2006, 97, 064502, DOI: 10.1103/PhysRevLett.97.064502, PMID: 17026172, Download


[2] P. Nemes, G. Schlosser, and K. Vekey, Amino acid cluster formation studied by electrospray ionization mass spectrometry, J. Mass Spectrom. 2005, 40, 4349, DOI: 10.1002/jms.771, PMID: 15584012, Download


[1] Z. Novak, P. Nemes, and A. Kotschy, Tandem Sonogashira coupling: An efficient tool for the synthesis of diarylalkynes, Org. Lett. 2004, 6, 49174920, DOI: 10.1021/ol047983f, PMID: 15606099, Download